Immunoaffinity Columns

Ontwsep™ Immunoaffinity Columns

Ontwsep™ Immunoaffinity Columns are designed for the separation and purification of mycotoxins from samples. The principle is based on the specific reaction between antigens and antibodies. Antibodies in the column bind target toxins, while other substances are washed away. After washing and elution, the purified toxins are collected for detection.

Features:

High Specificity: Efficiently identifies target substances, reducing interference and extending column life.

High Stability: RSD < 5%, meeting standard method requirements.

High Recovery Rate: Recovery rates between 90%–105%.

High Flow Efficiency: Stable flow rates minimize clogging.

Strong Enrichment Capacity: Allows large sample volumes for comprehensive target adsorption.

Wide Applicability: Complies with international standards and GB 5009 series for mycotoxin detection.

Relevant Standards

1. European Union (EU) Standards

EU Regulation No. 1881/2006: Sets maximum levels for certain contaminants, including mycotoxins, in foodstuffs.

EN 16923:2017: Foodstuffs – Determination of T-2 and HT-2 toxins in cereals and cereal-based products.

EN 17279:2019: Foodstuffs – Determination of zearalenone in food by HPLC with fluorescence detection.

EN 15851:2010: Foodstuffs – Determination of aflatoxin B1 in cereal-based foods for infants and young children.

 

2. United States (US) Standards

FDA Guidelines:

Aflatoxins: Action levels for aflatoxins in food and feed (e.g., 20 ppb for aflatoxin B1 in food).

Deoxynivalenol (DON): Advisory levels for DON in finished wheat products.

Ochratoxin A: Guidance for industry on ochratoxin A in coffee, wine, and grape juice.

 

AOAC Official Methods:

AOAC 2005.08: Determination of aflatoxins in animal feed by immunoaffinity column cleanup and HPLC.

AOAC 991.31: Determination of ochratoxin A in wine and beer.

 

3. Codex Alimentarius (International Food Standards)

Codex STAN 193-1995: General standard for contaminants and toxins in food and feed.

Codex CXS 193-1995: Maximum levels for aflatoxins, ochratoxin A, and other mycotoxins in various food commodities.

 

4. Japan Standards

Japanese Food Sanitation Law:

Sets maximum limits for aflatoxins (10 ppb total aflatoxins in foods).

Ochratoxin A and deoxynivalenol are also regulated in specific food categories.

Japanese Agricultural Standards (JAS): Includes guidelines for mycotoxin testing in agricultural products.

 

5. Australia and New Zealand Standards

Australia New Zealand Food Standards Code (Standard 1.4.1):

Sets maximum levels for aflatoxins, ochratoxin A, and patulin in food.

AS 5013.25-2009: Food microbiology – Examination for specific mycotoxins in cereals and cereal products.

 

6. Canada Standards

Canadian Food Inspection Agency (CFIA) Guidelines:

Maximum levels for aflatoxins, ochratoxin A, and deoxynivalenol in food and feed.

Health Canada’s Mycotoxin Regulations: Includes guidelines for mycotoxin testing in imported and domestic food products.

 

7. China Standards

GB 2761-2017: National food safety standard for limits of mycotoxins in foods.

Includes limits for aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, and T-2 toxin.

GB 5009 Series: Detailed analytical methods for mycotoxin detection in food.

 

8. India Standards

Food Safety and Standards Authority of India (FSSAI) Regulations:

Sets maximum limits for aflatoxins, ochratoxin A, and patulin in food products.

IS 5887 (Part 1): 2017: Methods for detection of aflatoxins in food and feed.

 

9. South Africa Standards

Regulations Relating to Mycotoxins in Foodstuffs (R. 1145):

Sets maximum limits for aflatoxins, ochratoxin A, and fumonisins in food.

 

10. Brazil Standards

ANVISA (Brazilian Health Regulatory Agency) Regulations:

Sets maximum limits for aflatoxins, ochratoxin A, and deoxynivalenol in food.

RDC No. 7/2011: Technical regulation on mycotoxin limits in food.

Applications

Food Safety Testing: Detection of mycotoxins in grains, nuts, dairy products, and spices. 

Agricultural Products: Monitoring mycotoxin contamination in crops and animal feed.

Regulatory Compliance: Ensuring adherence to national and international food safety standards.

Global Harmonization Efforts

Many countries align their mycotoxin regulations with Codex Alimentarius standards to facilitate international trade and ensure food safety. Additionally, organizations like the International Organization for Standardization (ISO) and AOAC International provide globally recognized methods for mycotoxin analysis.

Summary Table of Key Mycotoxin Limits

Mycotoxin	Commodity	EU Limit (ppb)	US Limit (ppb)	Codex Limit (ppb)	China Limit (ppb)
Aflatoxin B1	Cereals, nuts, dried fruits	2–12	20	15	5–20
Ochratoxin A	Cereals, coffee, wine	3–10	N/A	5–10	5–10
Deoxynivalenol (DON)	Cereals, cereal products	200–1750	1000	1000–2000	1000–2000
Zearalenone	Cereals, cereal products	20–100	N/A	50–100	50–100
T-2 and HT-2 Toxins	Cereals, cereal products	50–200	N/A	N/A	N/A

 

Ordering Information

Part Number	Product Description	Specification	Column Capacity	Detection Items
E01-01-03-25	Ontwsep™ Aflatoxins Total	3mL/pc, 25pc/Box	300ng	Cereals, oils, feed, herbs, condiments, tea, alcoholic beverages
E01-02-03-25	Ontwsep™ Aflatoxin B1	3mL/pc, 25pc/Box	300ng	Cereals, oils, feed, herbs, condiments, tea, alcoholic beverages
E01-03-03-25	Ontwsep™ Aflatoxin M1	3mL/pc, 25pc/Box	100ng	Milk, milk powder, cheese, and other dairy products
A01-04-03-25	Ontwsep™ Deoxynivalenol	3mL/pc, 25pc/Box	2000ng	Cereals, oils, feed, herbs, condiments, alcoholic beverages
E01-05-03-25	Ontwsep™ Zearalenone	3mL/pc, 25pc/Box	1500ng	Cereals, oils, feed, herbs, condiments, alcoholic beverages
E01-06-03-25	Ontwsep™ Ochratoxin A	3mL/pc, 25pc/Box	100ng	Cereals, oils, feed, herbs, condiments, alcoholic beverages
E01-07-03-25	Ontwsep™ Fumonisin	3mL/pc, 25pc/Box	5000ng	Cereals, oils, feed, herbs, condiments, alcoholic beverages
E01-08-03-25	Ontwsep™ Trichothecenes T-2	3mL/pc, 25pc/Box	2000ng	Cereals, oils, feed, herbs, condiments, alcoholic beverages
E02-01-03-50	Ontwsep™ VB12	3mL/pc, 50pc/Box	800ng	Health products, dietary supplements
E02-02-03-50	Ontwsep™ HP	3mL/pc, 25pc/Box	2mg	Dairy products, milk powder