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Determination of acetylglutamide-related substances by Excsep AQ-C18

  1. HPLC chromatographic conditions

Column: Excsep®  AQ-C18,4.6 mmx250 mm, 5 μm (P/N: EAQC18-5120-046250S); Column

Temperature: 25℃;

Mobile phase: 0.05 mol/L potassium dihydrogen phosphate solution (pH was adjusted to 3.0 with 10% phosphoric acid) -methanol (95:5);

Flow rate: 1.0 mL/min;

Detection wavelength: 210 nm;

Injection volume: 20 μL, and the chromatogram was recorded up to three times the retention time of the main component peak.

Please refer to the link below: https://acrobat.adobe.com/id/urn:aaid:sc:ap:ff70b251-a1ab-452b-9d96-753bef4acbd5

Updated on 15 May 2025