ExcsepTM Sugar Columns User Manual

OneTwo Chromatography Technology has specially developed analytical columns based on polymer microspheres to meet the needs of the food analysis and pharmaceutical industries for the analysis of polysaccharides (e.g. starch, cellulose, glucose, galactose, fibrous ditangles, mannitol, glycogen, etc.) and organic acids (e.g. acetic acid, oxalic acid, etc.).

Three types of columns are currently available: ExcsepTM Sugar-H, ExcsepTM Sugar-Ca, and ExcsepTM Sugar-Na.

Column Activation

Every new column must be activated before use. Improper activation may lead to issues such as for use often cause problems such as unstable baselines, reduced column efficiency, retention time drift, and poor separation and column performance.

ExcsepTM Sugar-H column activation was performed using aqueous sulfuric acid solution at pH=2.5, 0.5 mL/min flow rate, and flushing at 30°C~40°C for 12 hours.

ExcsepTM Sugar-Ca and ExcsepTM Sugar-Na columns were activated with water, 0.5 mL/min flow rate, and flushed at 30°C~40°C for 12 hours.

Mobile Phase

Column Temperature and Pressure Limits

ExcsepTM Sugar Series columns have a temperature limit of 95℃; pressure limit of 1200 psi.

Storage

ExcsepTM Sugar Series columns are recommended to be stored in water or mobile phase solution in a refrigerator at 4°C to avoid bacterial growth.

Regeneration

1. Positively connected column.
2. ExcsepTM Sugar-H column was flushed with aqueous sulfuric acid solution pH=2.5 at a flow rate of 0.5 mL/min for 12 hours at 80℃~85℃.

The ExcsepTM Sugar-Ca column was flushed with 0.5 g/L EDTA Ca (CAS: 23411-34-9) solution at a flow rate of 0.5 mL/min for 12 hr at 80°C~85°C. The column was used to analyze the chromatographic properties of the column.

The ExcsepTM Sugar-Na column was flushed with 0.1 mol/L sodium nitrate (CAS: 631-99-4) solution at a flow rate of 0.5 mL/min for 12 hours at 80℃~85℃.

1. Equilibrate using the desired mobile phase.